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Addgene

pcDNA5 HIS-Ub-K48R
(Plasmid #221368)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221368 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA5
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ubiquitin
  • Species
    H. sapiens (human)
  • Mutation
    Changed Lysine 48 to Arginine
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHIS (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5 HIS-Ub-K48R was a gift from Aga Gambus (Addgene plasmid # 221368 ; http://n2t.net/addgene:221368 ; RRID:Addgene_221368)

  • For your References section:

    Characterizing replisome disassembly in human cells. Jones RM, Ruiz JH, Scaramuzza S, Nath S, Liu C, Henklewska M, Natsume T, Bristow RG, Romero F, Kanemaki MT, Gambus A. iScience. 2024 Jun 12;27(7):110260. doi: 10.1016/j.isci.2024.110260. eCollection 2024 Jul 19. 10.1016/j.isci.2024.110260 PubMed 39055910
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