pLenti CMV E7 Hygro (w188-4)
(Plasmid
#22256)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 8733
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3, 37oC
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman papilloma virus E7
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Alt nameE7
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SpeciesHPV
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Insert Size (bp)296
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Entrez GeneE7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer CMVforw
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byE7 cDNA was obtained from Dr. Christian Beausejour.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The E7 protein binds and inactivates a variety of human proteins, notably the retinoblastoma tumor suppresor protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti CMV E7 Hygro (w188-4) was a gift from Eric Campeau (Addgene plasmid # 22256)