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pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122
(Plasmid #223186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 223186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    YAP5SA
  • Species
    M. musculus (mouse)
  • Mutation
    S61A, S109A, S127A, S164A, S381A
  • Entrez Gene
    Yap1 (a.k.a. Yap, Yap65, Yki, Yorkie)
  • Promoter TNNT2
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • engineered P2A (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGCATTCCGTCAGATACACAAAGTgctactaacttcagcctActAaaAcaggctggagacgtTgaggagaaccctggacctTATCCATAcGACGTTCCAGATTACGCagatcccgggcagcagc
  • 3′ sequencing primer ctagataaagaaagctttcttacatggttGTACCTCTAGGTACCTCTAGAGTCGACCC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mir122
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mir122 (a.k.a. Mir122a, Mir122b, Mirn122a, Mirn122b, mir-122, mmu-mir-122)
  • Promoter TNNT2

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atggttGTACCTCTAGGTACgatggggactgaaagcttcgg
  • 3′ sequencing primer CGTCCTCGAGGCCGCCCGGGcggtatcgataagctTcggcg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122 was a gift from Yuxuan Guo (Addgene plasmid # 223186 ; http://n2t.net/addgene:223186 ; RRID:Addgene_223186)

  • For your References section:

    The drug-elicitable alternative splicing module for tunable vector expression in the heart. Chen Z, Yang L, Zhang Y, Li J, Yang Y, Li Y, Fan L, Chen W, Miao L, Liu J, Chen G, Wang Z, Li Y, Gao F, Zhou J, Zheng L, Zhang Y, Zhao D, Pu WT, Yang K, Dong E, Guo Y. Nat Cardiovasc Res. 2025 Jun 13. doi: 10.1038/s44161-025-00665-7. 10.1038/s44161-025-00665-7 PubMed 40514436
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