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CaMKII-pHluorin-Synaptotagmin1
(Plasmid #224430)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224430 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CamKII-LGI1-pHluorin
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 2115
  • Total vector size (bp) 11354
  • Modifications to backbone
    Replace LGI1 with Synaptotagmin1
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Bleomycin resistance

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptotagmin1-pHluorin
  • Alt name
    Tagmin1-pH
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    11354
  • GenBank ID
    NM_25716
  • Promoter CaMKII
  • Tag / Fusion Protein
    • pHluorin

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.19.590083 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaMKII-pHluorin-Synaptotagmin1 was a gift from Jaime de Juan-Sanz (Addgene plasmid # 224430 ; http://n2t.net/addgene:224430 ; RRID:Addgene_224430)

  • For your References section:

    Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling. Pascual-Caro C, de Juan-Sanz J. PLoS Biol. 2024 Oct 28;22(10):e3002860. doi: 10.1371/journal.pbio.3002860. eCollection 2024 Oct. 10.1371/journal.pbio.3002860 PubMed 39466808
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