pISFba1
(Plasmid
#226820)
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PurposeExpressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 226820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameISFba1
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SpeciesFirmicutes bacterium AM43-11BH
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISFba1 was a gift from Sheng Yang (Addgene plasmid # 226820 ; http://n2t.net/addgene:226820 ; RRID:Addgene_226820) -
For your References section:
Implementation of RAGATH RNA-associated DNA Endonucleases as Genome Editing Tool in Escherichia coli. Zhou X, Yang S, Sun B, Dong F, Yin M, Jiang Y, Huang Z, Yang S. Biotechnol J. 2025 Mar;20(3):e70005. doi: 10.1002/biot.70005. 10.1002/biot.70005 PubMed 40111000