Skip to main content
Addgene

pTRIP-hPGK-Blast-2A-STING-mNeonGreen
(Plasmid #227186)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pTRIP
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STING1
  • Species
    H. sapiens (human)
  • Entrez Gene
    STING1 (a.k.a. ERIS, MITA, MPYS, NET23, SAVI, STING, STING-beta, TMEM173, hMITA, hSTING)
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gattcgtgaattgctgccct
  • 3′ sequencing primer atgctgattgtgcctggcta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.07.588166 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP-hPGK-Blast-2A-STING-mNeonGreen was a gift from Nir Hacohen (Addgene plasmid # 227186 ; http://n2t.net/addgene:227186 ; RRID:Addgene_227186)

  • For your References section:

    Classification and functional characterization of regulators of intracellular STING trafficking identified by genome-wide optical pooled screening. Gentili M, Carlson RJ, Liu B, Hellier Q, Andrews J, Qin Y, Blainey PC, Hacohen N. Cell Syst. 2024 Dec 18;15(12):1264-1277.e8. doi: 10.1016/j.cels.2024.11.004. Epub 2024 Dec 9. 10.1016/j.cels.2024.11.004 PubMed 39657680
Related items: