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Addgene

pB-tre-dCas9-VPR
(Plasmid #227506)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227506 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Vector type
    CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPR-dCas9
  • Alt name
    Blasticidin resistence
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Derived from Wysocka Lab plasmid PiggyBac dCas9-VPR (Addgene #191269) wih TagBFP removed

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-tre-dCas9-VPR was a gift from Joanna Wysocka (Addgene plasmid # 227506 ; http://n2t.net/addgene:227506 ; RRID:Addgene_227506)

  • For your References section:

    Long-range regulation of transcription scales with genomic distance in a gene-specific manner. Jensen CL, Chen LF, Swigut T, Crocker OJ, Yao D, Bassik MC, Ferrell JE Jr, Boettiger AN, Wysocka J. Mol Cell. 2025 Jan 16;85(2):347-361.e7. doi: 10.1016/j.molcel.2024.10.021. Epub 2024 Dec 2. 10.1016/j.molcel.2024.10.021 PubMed 39626660
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