YAP/TAZ tdTomato TEAD Binding Reporter
(Plasmid
#228554)
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PurposeEncodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLKO.1
- Backbone size w/o insert (bp) 7728
- Total vector size (bp) 8789
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdTomato
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SpeciesH. sapiens (human)
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Insert Size (bp)1431
- Promoter GTIIC TEAD-Binding Responsive Element
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cgaggtcgacggtatcg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YAP/TAZ tdTomato TEAD Binding Reporter was a gift from Cole DeForest (Addgene plasmid # 228554 ; http://n2t.net/addgene:228554 ; RRID:Addgene_228554) -
For your References section:
Stepwise Stiffening/Softening of and Cell Recovery from Reversibly Formulated Hydrogel Interpenetrating Networks. Kopyeva I, Goldner EC, Hoye JW, Yang S, Regier MC, Bradford JC, Vera KR, Bretherton RC, Robinson JL, DeForest CA. Adv Mater. 2024 Sep 6:e2404880. doi: 10.1002/adma.202404880. 10.1002/adma.202404880 PubMed 39240007