Skip to main content
Addgene

pET28a-UBC9-K14R-A129K
(Plasmid #228948)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228948 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5236
  • Total vector size (bp) 5758
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UBC9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    522
  • Mutation
    changed Lysine 14 to Arginine, changed Alanine 129 to Lysine
  • GenBank ID
    NM_194260.3
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)
  • Promoter T7-lac
  • Tag / Fusion Protein
    • His tag and thrombine cleavage site : MGSSHHHHHHSSGLVPRGSHM (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-UBC9-K14R-A129K was a gift from Marcin Suskiewicz (Addgene plasmid # 228948 ; http://n2t.net/addgene:228948 ; RRID:Addgene_228948)
Related items: