Skip to main content
Addgene

pU6-Osp.gRNA-MS2in
(Plasmid #229772)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229772 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pU6
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA cassette with two MS2 stem loops
  • gRNA/shRNA sequence
    gtttCAGAGCTAggccaacatgaggatcacccatgtctgcagggccTGCTGGAAACAGCATAGCAAGTTGaaataaggctagtccgttatcaacttggccaacatgaggatcacccatgtctgcagggccgaaaaagtggcaccgagtcggtgcTTTTTT
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-Osp.gRNA-MS2in was a gift from Yujia Cai (Addgene plasmid # 229772 ; http://n2t.net/addgene:229772 ; RRID:Addgene_229772)

  • For your References section:

    Customizable virus-like particles deliver CRISPR-Cas9 ribonucleoprotein for effective ocular neovascular and Huntington's disease gene therapy. Ling S, Zhang X, Dai Y, Jiang Z, Zhou X, Lu S, Qian X, Liu J, Selfjord N, Satir TM, Lundin A, Touza JL, Firth M, Van Zuydam N, Bilican B, Akcakaya P, Hong J, Cai Y. Nat Nanotechnol. 2025 Apr;20(4):543-553. doi: 10.1038/s41565-024-01851-7. Epub 2025 Feb 10. 10.1038/s41565-024-01851-7 PubMed 39930103
Related items: