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Addgene

pHW621
(Plasmid #229867)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229867 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pNM3469
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB® Turbo competent E. coli
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
  • Species
    C. elegans (nematode), Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The E. coli strain DH5α bacterial clone with this construct grows slowly, resulting in noticeably small numbers of colonies following overnight incubation. The DNA construct is likely toxic to the E. coli strain DH5α, resulting in unexpected random insertion or deletion. If this plasmid is transformed into DH5α competent cells. It is advisable to routinely sequence of the section of cGAL(DBD)-linker-QFAD-tbb-2 3'UTR following each fresh miniprep from a new bacterial culture to confirm absence of any mutations. To overcome this issue, the deposited bacterial stock of this plasmid is with NEB® Turbo competent E. coli, which tolerates this construct better and produces the plasmid with the correct sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHW621 was a gift from Han Wang (Addgene plasmid # 229867 ; http://n2t.net/addgene:229867 ; RRID:Addgene_229867)
  • For your References section:

    A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. Khan H, Huang X, Raj V, Wang H. PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan. 10.1371/journal.pgen.1011541 PubMed 39841730