pHW621
(Plasmid #229867)

• Purpose: Entry vector with cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR in slot 5 (for SapTrap assembly)
• Depositing Lab: Han Wang
• Publication: Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan.

Backbone

• Vector backbone: pNM3469
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB® Turbo competent E. coli
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
• Species: C. elegans (nematode), Synthetic

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pHW621

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The E. coli strain DH5α bacterial clone with this construct grows slowly, resulting in noticeably small numbers of colonies following overnight incubation. The DNA construct is likely toxic to the E. coli strain DH5α, resulting in unexpected random insertion or deletion. If this plasmid is transformed into DH5α competent cells. It is advisable to routinely sequence of the section of cGAL(DBD)-linker-QFAD-tbb-2 3'UTR following each fresh miniprep from a new bacterial culture to confirm absence of any mutations. To overcome this issue, the deposited bacterial stock of this plasmid is with NEB® Turbo competent E. coli, which tolerates this construct better and produces the plasmid with the correct sequence.