AiP2040 - pAAV-AiE2615m-SYFP2-WPRE3-BGHpA
(Plasmid
#230549)
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PurposeAiE2615m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 230549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AiP2040 - pAAV-AiE2615m-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Bosiljka Tasic (Addgene plasmid # 230549 ; http://n2t.net/addgene:230549 ; RRID:Addgene_230549) -
For your References section:
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types. Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D, Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N, Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, Levi BP, Ngai J, Ting JT, Tasic B. Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002. 10.1016/j.cell.2025.05.002 PubMed 40403729