pRSFDuet-SUMO-h-cGAS(T321I)
(Plasmid
#232294)
-
PurposeExpression of mutant human cGAS protein (T321I)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 232294 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepRSFDuet
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameMB21D1
-
MutationT321I
-
Entrez GeneCGAS (a.k.a. C6orf150, D4, MB21D1, h-cGAS)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRSFDuet-SUMO-h-cGAS(T321I) was a gift from Thomas Tuschl (Addgene plasmid # 232294 ; http://n2t.net/addgene:232294 ; RRID:Addgene_232294) -
For your References section:
Structural insight into the cGAS active site explains differences between therapeutically relevant species. Skeldon AM, Wang L, Sgarioto N, Beveridge RE, Chan S, Dorich S, Dumais V, Fradet N, Gaudreault S, LeGros P, McKay D, Seliniotakis R, Sietsema DV, Zhang L, Boily MO, Burch JD, Caron A, Fader LD, Lama L, Xie W, Patel DJ, Tuschl T, Crackower MA, Pike KA. Commun Chem. 2025 Mar 22;8(1):88. doi: 10.1038/s42004-025-01481-7. 10.1038/s42004-025-01481-7 PubMed 40121343