pAAV-ATLASsnCre
(Plasmid #232351)

• Purpose: Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.
• Depositing Lab: Don Arnold
• Publication: ATLAS: A rationally designed anterograde transsynaptic tracer (unpublished)

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Backbone

• Vector backbone: pAAV_hsyn
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATLASsnCre
• Species: Synthetic
• Insert Size (bp): 2418
• Promoter: hSyn
• Tag / Fusion Protein:
  • ALFA-Tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: hSyn_F

Resource Information

• Supplemental Documents:
  • pAAV-hSyn-ATLASsnCre.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.12.557425 for bioRxiv preprint.