ubbr:GCaMP8m
(Plasmid
#232485)
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PurposeTo make fish transgenic lines that have calcium sensor expression in the whole body of the fish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232485 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonetol2
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namejGCaMP8m
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SpeciesD. rerio (zebrafish)
- Promoter ubbr
Cloning Information
- Cloning method Other
- 5′ sequencing primer X
- 3′ sequencing primer X (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFrom Dr.s Darius Balciunas and Justas Lazutka
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.03.21.586128 for bioRxiv preprint.
ubbr:GCaMP8m (ZT013)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ubbr:GCaMP8m was a gift from Chie Satou (Addgene plasmid # 232485 ; http://n2t.net/addgene:232485 ; RRID:Addgene_232485)