pGNL2-#18
(Plasmid
#232818)
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PurposeMammalian expression of His-tagged GNL2 (641-731aa)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepEGFP-C2
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Modifications to backboneNo EGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNL2 fragment (641-731aa)-6x His
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SpeciesH. sapiens (human)
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Mutationdeletion (1-640aa)
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Entrez GeneGNL2 (a.k.a. HUMAUANTIG, NGP1, Ngp-1, Nog2, Nug2)
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains a silent mutation in the codon for K641 in pGNL2 (AAG codon compared to AAA in the wild type sequence).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGNL2-#18 was a gift from Shiro Iuchi (Addgene plasmid # 232818)