pAAV2/5
(Plasmid
#232922)
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PurposeAAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232922 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * | |
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Backbone
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Vector backbonepUC19 with additional elements
- Total vector size (bp) 7373
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRep2/Cap5
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SpeciesParvovirus
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NOTE: This is an improved version of Addgene Plasmid #104964. Addgene recommends using this plasmid instead, as it consistently yields higher titers.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV2/5 was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 232922 ; http://n2t.net/addgene:232922 ; RRID:Addgene_232922)
