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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a
(Plasmid #233079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PX458
  • Backbone manufacturer
    Feng Zhang Lab
  • Backbone size w/o insert (bp) 2978
  • Total vector size (bp) 8937
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    5959
  • Promoter hU6, CBh
  • Tag / Fusion Protein
    • 2xFLAG

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a was a gift from Kyoungmi Kim (Addgene plasmid # 233079 ; http://n2t.net/addgene:233079 ; RRID:Addgene_233079)

  • For your References section:

    Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1. Park SJ, Ju S, Jung WJ, Jeong TY, Yoon DE, Lee JH, Yang J, Lee H, Choi J, Kim HS, Kim K. Nat Commun. 2025 Jan 15;16(1):677. doi: 10.1038/s41467-025-56048-w. 10.1038/s41467-025-56048-w PubMed 39809780
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