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pAK208_2xBRD9BD_Y57A-IFP2.0C
(Plasmid #233733)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 233733 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRD9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    766
  • Mutation
    Bromodomain cloned twice in tandem with Y57A mutation
  • Entrez Gene
    BRD9 (a.k.a. LAVS3040, PRO9856, SMARCI2)
  • Promoter CMV
  • Tag / Fusion Protein
    • IFP2.0 C-terminal part (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid 215750

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Exchange of 2xHP1bCD insert by 2xBRD9BD and introduce Y57A mutation.

Please visit https://doi.org/10.1101/2025.02.10.637448 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAK208_2xBRD9BD_Y57A-IFP2.0C was a gift from Albert Jeltsch (Addgene plasmid # 233733 ; http://n2t.net/addgene:233733 ; RRID:Addgene_233733)

  • For your References section:

    Development of a BiAD sensor for locus-specific detection of cellular histone acetylation dynamics by fluorescence microscopy. Köhler AR, Gutekunst N, Harsch A, Bashtrykov P, Jeltsch A. bioRxiv 2025.02.10.637448 10.1101/2025.02.10.637448
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