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UbC Puro ABL FRET V468F
(Plasmid #235035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235035 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUltra
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCR-ABL with internal StayGold and mScarlet3 tags
  • Species
    H. sapiens (human)
  • Mutation
    V468F
  • Entrez Gene
    ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
  • Promoter human ubiquitin C
  • Tag / Fusion Protein
    • PuroR-P2A (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mStayGold and mScarlet3 FRET pair measure ABL conformation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbC Puro ABL FRET V468F was a gift from Justin Pritchard (Addgene plasmid # 235035 ; http://n2t.net/addgene:235035 ; RRID:Addgene_235035)

  • For your References section:

    Dual functional genomics reveals a broad and convergent landscape of asciminib resistance in BCR::ABL1. Sokirniy I, Pritchard J. Genome Med. 2026
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