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Human Wild-type ASAP1
(Plasmid #235212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 6361
  • Total vector size (bp) 10000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ASAP1
  • Alt name
    ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6361
  • GenBank ID
    NM_001247996.2
  • Entrez Gene
    ASAP1 (a.k.a. AMAP1, CENTB4, DDEF1, PAG2, PAP, ZG14P)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Wild-type ASAP1 was a gift from Amy Shiu Lee (Addgene plasmid # 235212 ; http://n2t.net/addgene:235212 ; RRID:Addgene_235212)

  • For your References section:

    Endoplasmic reticulum stress activates SRC, relocating chaperones to the cell surface where GRP78/CD109 blocks TGF-beta signaling. Tsai YL, Ha DP, Zhao H, Carlos AJ, Wei S, Pun TK, Wu K, Zandi E, Kelly K, Lee AS. Proc Natl Acad Sci U S A. 2018 May 1;115(18):E4245-E4254. doi: 10.1073/pnas.1714866115. Epub 2018 Apr 13. 10.1073/pnas.1714866115 PubMed 29654145
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