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p3HM1Luc4
(Plasmid #236277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Modifications to backbone
    The MCS of pcDNA3 vector was modified
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    muscarinic M1 receptor
  • Alt name
    clone p3HM1Luc4
  • Species
    H. sapiens (human)
  • Entrez Gene
    CHRM1 (a.k.a. HM1, M1, M1R)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xHA (N terminal on insert)
    • Rluc8 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The MCS of the pcDNA3 vector was modified, so the structure of insert is: EcoRI - AsiSI - Start 3xHA-D2R - SbfI - RLuc8 - Stop AscI - XbaI - PacI - HindIII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3HM1Luc4 was a gift from Vsevolod Gurevich (Addgene plasmid # 236277 ; http://n2t.net/addgene:236277 ; RRID:Addgene_236277)

  • For your References section:

    Manipulation of very few receptor discriminator residues greatly enhances receptor specificity of non-visual arrestins. Gimenez LE, Vishnivetskiy SA, Baameur F, Gurevich VV. J Biol Chem. 2012 Aug 24;287(35):29495-505. doi: 10.1074/jbc.M112.366674. Epub 2012 Jul 11. 10.1074/jbc.M112.366674 PubMed 22787152
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