Skip to main content
Addgene
Open Menu

pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2
(Plasmid #236729)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 236729 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pJR103
  • Backbone manufacturer
    Marco Jost and Jonathan Weissman, Addgene plasmid #187242
  • Backbone size (bp) 9527
  • Modifications to backbone
    pJR103 was digested with XbaI and XhoI and ligated with "empty vector fragment" via NEBuilder.
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.19.639160 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2 was a gift from Derek Narendra (Addgene plasmid # 236729 ; http://n2t.net/addgene:236729 ; RRID:Addgene_236729)

  • For your References section:

    A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy. Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM, Sekine S, Li Y, Ward ME, Narendra DP. EMBO J. 2025 Nov 20. doi: 10.1038/s44318-025-00604-z. 10.1038/s44318-025-00604-z PubMed 41266657
Related items: