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Leishmania mexicana Cytosine Base Editing v1 Library
(Pooled Library #236783)

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  • Purpose

    Leishmania mexicana Cytosine Base Editing v1 (LmexBE_V1) is a genome-wide loss-of-function CRISPR base editing library designed to introduce STOP codons within the first 50% of coding sequences in the genome of L. mexicana. Libraries are transfected using the technique described in Herrmann-May, 2025 (Link opens in a new window). The library can be used for a range of screens in L. mexicana, such as fitness and drug resistance screens.

  • Vector Backbone

    pTB105 (Plasmid #236778) - does not express a base editor

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 236783 LmexBE_v1 pooled library† 1 $594 Add to Cart
Available to Academic and Nonprofits Only

  • † A stable cell line is required for the screen, expressing a Cas9 base editor, a Cas12a nuclease, and a T7 RNA Polymerase. Can be used in conjunction with either pTB106 or pTB107, they differ only in the type of base editor that is expressed. Recommended plasmids that allow for the expression of the required proteins include pTB106-Hyg (Addgene #236779), pTB106-Phleo (Addgene #236780), pTB107-Hyg (Addgene #236781), or pTB107-Phleo (Addgene #236782).

Library Details

  • Species
    Leishmania mexicana
  • Genes targeted
    7,936 genes or gene arrays (94% of the coding genome)
  • gRNAs per element
    1–6 (5.2 sgRNAs/gene on average)
  • gRNAs
    39,422
  • Controls
    1,000

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    25 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LmexBE_v1 library was a gift from Tom Beneke (Addgene #236783 ; http://n2t.net/addgene:236783 ; RRID:Addgene_236783)

  • For your References section:

    Genome-wide CRISPR base-editing screening defines drug response networks in Leishmania. Arias del Angel J, Link F, Herrmann May N, Ekici I, Wawra K, Schwind S, Zorn S, Haggarty J, Weidt SK, Ritchie R, Barrett MP, van Zandbergen G, Beneke T. bioRxiv 2025.06.16.659792. doi: 10.1101/2025.06.16.659792
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