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Addgene

TTR C10S/S85C/A120S
(Plasmid #238113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238113 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29a
  • Backbone manufacturer
    emdmillipore
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 5758
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    387
  • Mutation
    C10S/S85C/A120S
  • Entrez Gene
    TTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
  • Promoter T7

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We made mutations on the wild type construct received from the Jeffery Kelly lab. Description of wild type TTR plasmid in Zhang et al. 2014 ACS Chem Biol (https://doi.org/10.1021/cb5004477).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TTR C10S/S85C/A120S was a gift from Peter Wright (Addgene plasmid # 238113 ; http://n2t.net/addgene:238113 ; RRID:Addgene_238113)

  • For your References section:

    Mispacking of the F87 sidechain drives aggregation-promoting conformational fluctuations in the subunit interfaces of the transthyretin tetramer. Sun X, Ferguson JA, Yang K, Stanfield RL, Dyson HJ, Wright PE. Protein Sci. 2024 Sep;33(9):e5101. doi: 10.1002/pro.5101. 10.1002/pro.5101 PubMed 39149996
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