pLX_305_OVA_Blasticidin
(Plasmid
#238124)
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PurposeEnables expression of Ovalbumin with Blasticidin selectable marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLX_305
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Backbone manufacturerBroad Institute Genetic Perturbation Platform
- Backbone size w/o insert (bp) 7408
- Total vector size (bp) 8569
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOvalbumin
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SpeciesG. gallus (chicken)
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Insert Size (bp)1161
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GenBank ID396058
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Entrez GeneOVAL (a.k.a. OVA, SERPINB14)
- Promoter Human PGK
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CAATAGCGGCTGCTCAGCA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript performed this cloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX_305_OVA_Blasticidin was a gift from Arlene Sharpe (Addgene plasmid # 238124 ; http://n2t.net/addgene:238124 ; RRID:Addgene_238124)