pRK5_CKAR3
(Plasmid
#238411)
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PurposeMammalian expression of CKAR3 under a CMV promotor.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
- Backbone size w/o insert (bp) 4760
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCKAR3 (PKC sensor)
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SpeciesSynthetic
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Insert Size (bp)2025
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5_CKAR3 was a gift from Haining Zhong (Addgene plasmid # 238411 ; http://n2t.net/addgene:238411 ; RRID:Addgene_238411) -
For your References section:
A high-performance genetically encoded sensor for cellular imaging of PKC activity in vivo. Yahiro T, Bayless-Edwards L, Jones JA, Ma L, Qin M, Mao T, Zhong H. bioRxiv [Preprint]. 2024 Jul 23:2024.07.19.604387. doi: 10.1101/2024.07.19.604387. 10.1101/2024.07.19.604387 PubMed 39091834