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Addgene

pcDNA-ApplePy1Low
(Plasmid #239066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ApplePy1Low
  • Species
    Synthetic
  • Insert Size (bp)
    1272

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit ttps://doi.org/10.1101/2025.04.17.649293 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-ApplePy1Low was a gift from Robert Campbell (Addgene plasmid # 239066 ; http://n2t.net/addgene:239066 ; RRID:Addgene_239066)

  • For your References section:

    High-performance genetically-encoded green and red fluorescent biosensors for pyruvate. Imai S, Hario S, Suerte C, Yamaguchi I, Sakoi K, Terai T, Takahashi-Yamashiro K, Campbell RE. bioRxiv 2025.04.17.649293 10.1101/2025.04.17.649293
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