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pET28_Cas9d_Plasmid
(Plasmid #239445)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239445 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Twist Biosciences
  • Backbone size w/o insert (bp) 5682
  • Total vector size (bp) 7929
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9d
  • Alt name
    MG 34-1
  • Species
    Unknown
  • Insert Size (bp)
    2247
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_Cas9d_Plasmid was a gift from David Taylor (Addgene plasmid # 239445 ; http://n2t.net/addgene:239445 ; RRID:Addgene_239445)

  • For your References section:

    DNA targeting by compact Cas9d and its resurrected ancestor. Ocampo RF, Bravo JPK, Dangerfield TL, Nocedal I, Jirde SA, Alexander LM, Thomas NC, Das A, Nielson S, Johnson KA, Brown CT, Butterfield CN, Goltsman DSA, Taylor DW. Nat Commun. 2025 Jan 7;16(1):457. doi: 10.1038/s41467-024-55573-4. 10.1038/s41467-024-55573-4 PubMed 39774105
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