ATP5F1B-turboGFP
(Plasmid
#239795)
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PurposeExpresses human ATP5F1B tagged with turboGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV6-AC-GFP
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Backbone manufacturerOrigene
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 8096
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATP5F1b
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Alt nameATP5B
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SpeciesH. sapiens (human)
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Insert Size (bp)1583
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GenBank IDNM_001686
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Entrez GeneATP5F1B (a.k.a. ATP5B, ATPMB, ATPSB, HEL-S-271, HUMOP2)
- Promoter CMV
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Tag
/ Fusion Protein
- turboGFP (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ATP5F1B-turboGFP was a gift from Samantha Lewis (Addgene plasmid # 239795 ; http://n2t.net/addgene:239795 ; RRID:Addgene_239795) -
For your References section:
In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment. Rose K, Herrmann E, Kakudji E, Lizarrondo J, Celebi AY, Wilfling F, Lewis SC, Hurley JH. bioRxiv [Preprint]. 2025 Mar 25:2025.03.24.645001. doi: 10.1101/2025.03.24.645001. 10.1101/2025.03.24.645001 PubMed 40196634