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Addgene

pHS1742
(Plasmid #239838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239838 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OrufIscB-KRK
  • Species
    Human gut metagenome assembly
  • Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E409R/I533K
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • Nucleoplasmin NLS-3xHA (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS1742 was a gift from Feng Zhang (Addgene plasmid # 239838 ; http://n2t.net/addgene:239838 ; RRID:Addgene_239838)

  • For your References section:

    Evolution-guided protein design of IscB for persistent epigenome editing in vivo. Kannan S, Altae-Tran H, Zhu S, Xu P, Strebinger D, Oshiro R, Faure G, Moeller L, Pham J, Mears KS, Ni HM, Macrae RK, Zhang F. Nat Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3. 10.1038/s41587-025-02655-3 PubMed 40335752
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