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pIL6-PMEL-hbb
(Plasmid #239868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3Control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3636
  • Total vector size (bp) 5821
  • Modifications to backbone
    Introduction of multiple cloning site at 3' end of luciferase gene.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
  • Alt name
    PMEL
  • Alt name
    gp100
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2170
  • Mutation
    Valine to Glycine change is at amino acid 26 of nascent protein. This results in immunogenic KVP to KGP change at N-terminal of mature protein, immediately downstream of signal peptide.
  • GenBank ID
    NM_006928
  • Entrez Gene
    PMEL (a.k.a. D12S53E, HMB-45, HMB45, ME20, ME20-M, ME20M, P1, P100, PMEL17, SI, SIL, SILV, gp100)
  • Promoter T7 (included as part of insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Designed as a Gene Block based on NCBI sequence.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIL6-PMEL-hbb was a gift from Catherine Jopling (Addgene plasmid # 239868 ; http://n2t.net/addgene:239868 ; RRID:Addgene_239868)

  • For your References section:

    A proximity labelling-based approach to directly detect mRNA delivery to specific subcellular locations. Smart AD, Hughes ME, Ruiz Velasco AD, Hori N, Stolnik S, Jopling CL. Molecular Therapy Nucleic Acids, 102602. doi: 10.1016/j.omtn.2025.102602 10.1016/j.omtn.2025.102602
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