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Addgene

P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast
(Plasmid #239972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PiggyBac
  • Backbone size (bp) 7500
  • Modifications to backbone
    Mutate BsaI sites in the backbone
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Blasticidin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast was a gift from Jay Shendure (Addgene plasmid # 239972 ; http://n2t.net/addgene:239972 ; RRID:Addgene_239972)

  • For your References section:

    Symbolic recording of signalling and cis-regulatory element activity to DNA. Chen W, Choi J, Li X, Nathans JF, Martin B, Yang W, Hamazaki N, Qiu C, Lalanne JB, Regalado S, Kim H, Agarwal V, Nichols E, Leith A, Lee C, Shendure J. Nature. 2024 Aug;632(8027):1073-1081. doi: 10.1038/s41586-024-07706-4. Epub 2024 Jul 17. 10.1038/s41586-024-07706-4 PubMed 39020177
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