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pCAG-Calmodulin1 (CaM)
(Plasmid #240202)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240202 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4815
  • Total vector size (bp) 5262
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    calmodulin-1
  • Alt name
    CaM
  • Alt name
    Calm1
  • Alt name
    calmodulin-1 isoform 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    447
  • GenBank ID
    NM_009790.5 NM_009790.5
  • Entrez Gene
    Calm1 (a.k.a. CaM, Calm, Cam1)
  • Promoter pCAG
  • Tag / Fusion Protein
    • None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer GCCTCTGCTAACCATGTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-Calmodulin1 (CaM) was a gift from Ryohei Yasuda (Addgene plasmid # 240202 ; http://n2t.net/addgene:240202 ; RRID:Addgene_240202)

  • For your References section:

    Dendritic, delayed, stochastic CaMKII activation in behavioural time scale plasticity. Jain A, Nakahata Y, Pancani T, Watabe T, Rusina P, South K, Adachi K, Yan L, Simorowski N, Furukawa H, Yasuda R. Nature. 2024 Oct 9. doi: 10.1038/s41586-024-08021-8. 10.1038/s41586-024-08021-8 PubMed 39385027
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