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pvPE-V4
(Plasmid #240288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240288 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pvPE-V4
  • Alt name
    Cas9(H840A)-PERV-C RT(D199N, T305K, W312F, V222A, Y63R, C408R)-NCΔH-La
  • Species
    Synthetic; Cas9 is from S. pyogenes; PERV-C RT is derived from C subtype porcine endogenous retroviruses; La is from Homo sapiens
  • Insert Size (bp)
    6738
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on backbone)
    • SV40 NLS (C terminal on insert)
    • c-Myc NLS (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pvPE-V4 was a gift from Lusheng Huang (Addgene plasmid # 240288 ; http://n2t.net/addgene:240288 ; RRID:Addgene_240288)

  • For your References section:

    Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase. Liu W, Duan W, Peng Z, Liao Y, Wang X, Liu R, Jing Q, Jiang H, Fan Y, Ge L, Huang L, Xing Y. Trends Biotechnol. 2025 Aug 30:S0167-7799(25)00314-2. doi: 10.1016/j.tibtech.2025.07.029. 10.1016/j.tibtech.2025.07.029 PubMed 40885667
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