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pCMV-MMLVgag-D3A-3L-dCas9-ZIM3
(Plasmid #240531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240531 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDNA3-like
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 13789
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MMLVgag-D3A-3L-dCas9-ZIM3
  • Species
    Synthetic
  • Insert Size (bp)
    9027
  • Tag / Fusion Protein
    • FLAG, HA

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    MMLVgag is from pCMV-MMLVgag-3xNES-ABE8e (Plasmid #181751), DNMT3A-3L-dCas9 is from CRISPRoff-v2.1 (Plasmid #167981), and ZIM3 sequence was obtained from previous study (Alerasool, N., Segal, D., Lee, H. & Taipale, M. An efficient KRAB domain for CRISPRi applications in human cells. Nat Methods 17, 1093–1096 (2020).) and synthesized.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-MMLVgag-D3A-3L-dCas9-ZIM3 was a gift from James Nuñez (Addgene plasmid # 240531 ; http://n2t.net/addgene:240531 ; RRID:Addgene_240531)

  • For your References section:

    Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins. Xu D, Besselink S, Ramadoss GN, Dierks PH, Lubin JP, Pattali RK, Brim JI, Christenson AE, Colias PJ, Ornelas IJ, Nguyen CD, Chasins SE, Conklin BR, Nunez JK. bioRxiv [Preprint]. 2024 Nov 28:2024.11.26.625496. doi: 10.1101/2024.11.26.625496. 10.1101/2024.11.26.625496 PubMed 39651312
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