pUC19-SARS-CoV-2-N-gene-truncated
(Plasmid
#240635)
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PurposepUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains the SARS-CoV-2 N-gene and can be used as a positive control in SARS-CoV-2 research or diagnostics assays targeting the N gene.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
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Backbone manufacturerJoachim Messing, Addgene plasmid #50005
- Backbone size w/o insert (bp) 2367
- Total vector size (bp) 2457
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Modifications to backbonenone
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2-N-gene-truncated
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Insert Size (bp)86
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Entrez GeneN (a.k.a. GU280_gp10)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAlexandra R. Bender (co-author) cloned the gene.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-SARS-CoV-2-N-gene-truncated was a gift from Kevin Yehl (Addgene plasmid # 240635 ; http://n2t.net/addgene:240635 ; RRID:Addgene_240635)
Map uploaded by the depositor.
