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pGEX-OGTc(R989A)
(Plasmid #24115)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24115 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone size w/o insert (bp) 4948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OGT
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3000
  • Mutation
    R989A
  • Entrez Gene
    Ogt
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was initially cloned by Xiaoyong Yang, currently at Yale.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-OGTc(R989A) was a gift from Ronald Evans (Addgene plasmid # 24115)

  • For your References section:

    Phosphoinositide signalling links O-GlcNAc transferase to insulin resistance. Yang X, Ongusaha PP, Miles PD, Havstad JC, Zhang F, So WV, Kudlow JE, Michell RH, Olefsky JM, Field SJ, Evans RM. Nature. 2008 Feb 21. 451(7181):964-9. 10.1038/nature06668 PubMed 18288188
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