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tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry
(Plasmid #241314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241314 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTRE2
  • Total vector size (bp) 5769
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Adar deaminase domain
  • Species
    M. musculus (mouse)
  • Mutation
    E957Q
  • Entrez Gene
    Adar (a.k.a. Adar1, Adar1p110, Adar1p150, DRADA, mZaADAR)
  • Promoter tetO and CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The "HyperTRIBE" mutation is E488Q in drosophila and is conserved at the E957Q amino acid in the mouse Adar gene (amino acid references transcript uc008pzx.2, which is 1178 amino acids in length). This insert is a C-terminal truncation of the total Adar gene from T788 to F1170 - which spans the deaminase domain.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetO-HyperTRIBE-adar_deaminase-V5-t2a-mCherry was a gift from Stavros Lomvardas (Addgene plasmid # 241314 ; http://n2t.net/addgene:241314 ; RRID:Addgene_241314)
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