Lenti NG-ABE8e_P2A_GFP + PuroR
(Plasmid
#242000)
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PurposeLentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneAdapted from Addgene plasmid #110869, which was based on lentiCRISPRv1
- Backbone size w/o insert (bp) 8400
- Total vector size (bp) 13171
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNG-ABE8e
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Alt nameNG-Cas9-ABE8e adenine base editor, adapted from Addgene plasmid #138491
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Insert Size (bp)4815
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti NG-ABE8e_P2A_GFP + PuroR was a gift from Frank Buchholz (Addgene plasmid # 242000 ; http://n2t.net/addgene:242000 ; RRID:Addgene_242000)
Map uploaded by the depositor.
