pFN1-Aad9
(Plasmid
#244449)
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PurposeA shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 244449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2720
- Total vector size (bp) 4109
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namepFn1 Fn ori
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SpeciesSynthetic
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Insert Size (bp)546
Gene/Insert 2
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Gene/Insert nameAad9
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Alt nameaad9-opt-Fn
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SpeciesSynthetic
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Insert Size (bp)753
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.07.17.603877 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFN1-Aad9 was a gift from Xue Liu (Addgene plasmid # 244449 ; http://n2t.net/addgene:244449 ; RRID:Addgene_244449)