pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mRuby3
(Plasmid
#244912)
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PurposeiATPSnFR2.A95K variant with non-responsive mRuby3
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 244912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameiATPSnFR2.A95K.mRuby3
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SpeciesM. musculus (mouse)
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MutationA95K
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.SynFLEX.(cyto).iATPSnFR2.A95K.mRuby3 was a gift from Tim Brown & HHMI-JRC Tool Translation Team (Addgene plasmid # 244912 ; http://n2t.net/addgene:244912 ; RRID:Addgene_244912) -
For your References section:
iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring intracellular ATP. Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown TA, Ryan TA. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 10.1073/pnas.2314604121. Epub 2024 May 15. 10.1073/pnas.2314604121 PubMed 38748581