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Rab3a Biosensor
(Plasmid #245019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245019 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab3a
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Rab3a (a.k.a. RAB3)
  • Tag / Fusion Protein
    • Biosensor (mScarlet-I/NeonGreen) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences. Addgene NGS finds Q81 (not Q81L) in Rab3a (NP_037150.2), several silent mutations in mNeonGreen, and discrepancies in linker sequences in the insert ORF.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rab3a Biosensor was a gift from Thomas Martin (Addgene plasmid # 245019 ; http://n2t.net/addgene:245019 ; RRID:Addgene_245019)
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