pUC19_PHF19_HTC3xF_BSD_donor
(Plasmid
#246398)
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PurposePHF19 donor plasmid with blasticidin resistance
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246398 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePHF19
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SpeciesH. sapiens (human)
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Entrez GenePHF19 (a.k.a. MTF2L1, PCL3, TDRD19B)
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Tag
/ Fusion Protein
- HaloTag_3XFLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19_PHF19_HTC3xF_BSD_donor was a gift from Yicheng Long (Addgene plasmid # 246398 ; http://n2t.net/addgene:246398 ; RRID:Addgene_246398) -
For your References section:
Distinct specificity and functions of PRC2 subcomplexes in human stem cells and cardiac differentiation. Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y. Mol Cell. 2025 Aug 7:S1097-2765(25)00615-X. doi: 10.1016/j.molcel.2025.07.014. 10.1016/j.molcel.2025.07.014 PubMed 40803325