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pCDH-EF1-E-'No'Mi-Red-T2A-Puro
(Plasmid #246642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 246642 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCDH, from Addgene Plasmid #207805
  • Backbone manufacturer
    Breyne Lab
  • Backbone size w/o insert (bp) 2133
  • Total vector size (bp) 10881
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cleavable E-NoMi-Red
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2781
  • Promoter EF-1alpha
  • Tags / Fusion Proteins
    • FLAG-Nanoluciferase (N terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-E-'No'Mi-Red-T2A-Puro was a gift from Koen Breyne (Addgene plasmid # 246642 ; http://n2t.net/addgene:246642 ; RRID:Addgene_246642)

  • For your References section:

    Engineering of CD63 Enables Selective Extracellular Vesicle Cargo Loading and Enhanced Payload Delivery. Obuchi W, Zargani-Piccardi A, Leandro K, Rufino-Ramos D, Di Lanni E, Frederick DM, Maalouf K, Nieland L, Xiao T, Repiton P, Vaine CA, Kleinstiver BP, Bragg DC, Lee H, Miller MA, Breakefield XO, Breyne K. J Extracell Vesicles. 2025 Jun;14(6):e70094. doi: 10.1002/jev2.70094. 10.1002/jev2.70094 PubMed 40527733
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