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pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA
(Plasmid #247646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247646 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPB-PGKneo
  • Backbone size w/o insert (bp) 6661
  • Total vector size (bp) 8995
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MARIO
  • Species
    Synthetic
  • Insert Size (bp)
    2025
  • GenBank ID
    LC316970
  • Tag / Fusion Protein
    • NLS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA was a gift from Takeharu Nagai (Addgene plasmid # 247646 ; http://n2t.net/addgene:247646 ; RRID:Addgene_247646)

  • For your References section:

    A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T. Curr Biol. 2018 Feb 5;28(3):444-451.e6. doi: 10.1016/j.cub.2017.12.035. Epub 2018 Jan 18. 10.1016/j.cub.2017.12.035 PubMed 29358072
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