pAAV-DIO-DREADD-Gs
(Plasmid
#247959)
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PurposeCre dependant viral expression of HA-DREADD-Gs
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 247959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDREADD-Gs
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer ggacggcccttctcctccggg
- 3′ sequencing primer GTAATCCAGAGGTTGATTATCG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-DIO-DREADD-Gs was a gift from Giovanni Marsicano (Addgene plasmid # 247959 ; http://n2t.net/addgene:247959 ; RRID:Addgene_247959) -
For your References section:
Potentiation of mitochondrial function by mitoDREADD-G(s) reverses pharmacological and neurodegenerative cognitive impairment in mice. Pagano Zottola AC, Martin-Jimenez R, Lavanco G, Hamel-Cote G, Ramon-Duaso C, Rodrigues RS, Mariani Y, Khan M, Drago F, Jean S, Rio IB, Jimenez-Blasco D, Egana-Huguet J, Eraso-Pichot A, Beriain S, Cannich A, Vidal-Palencia L, Infantino R, Julio-Kalajzic F, Gisquet D, Goncalves A, Al-Younis I, Baussan Y, Duvezin-Caubet S, Devin A, Soria-Gomez E, Puente N, Bolanos JP, Grandes P, Pouvreau S, Busquets-Garcia A, Marsicano G, Bellocchio L, Hebert-Chatelain E. Nat Neurosci. 2025 Sep;28(9):1844-1857. doi: 10.1038/s41593-025-02032-y. Epub 2025 Aug 11. 10.1038/s41593-025-02032-y PubMed 40790270