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pCX:Gachapin-4B-P2A-EBFP2-NLS
(Plasmid #251134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251134 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCX
  • Backbone size w/o insert (bp) 4692
  • Total vector size (bp) 7251
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gachapin-4B
  • Species
    Synthetic
  • Insert Size (bp)
    2559
  • Promoter Chicken β-actin promoter
  • Tag / Fusion Protein
    • P2A-EBFP2-NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CTTCTGGCGTGTGACCGG
  • 3′ sequencing primer GCTTCATGATGTCCCCAT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kanadome et al. Commun Biol. 2022. doi: 10.1038/s42003-022-04023-2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX:Gachapin-4B-P2A-EBFP2-NLS was a gift from Takeharu Nagai (Addgene plasmid # 251134 ; http://n2t.net/addgene:251134 ; RRID:Addgene_251134)

  • For your References section:

    Fluorescent indicators for visualizing dynamic contact between cells and between processes originating from a single cell. Kanadome T, Hoshino N, Jitsuki S, Hashimoto H, Yagi T, Nagai T. Cell Rep Methods. 2026 Jan 23:101292. doi: 10.1016/j.crmeth.2025.101292. 10.1016/j.crmeth.2025.101292 PubMed 41579865
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