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4784 TRV2-eTnpBc-reRNA4PDS
(Plasmid #251816)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 251816 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    T-DNA
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Enhanced variant of ISDra2 eTnpBc with guide targeting NbPDS with HDV at 3' end
  • Alt name
    reRNA4 guide target: GCTACAATGAAGGAACTAGC
  • Species
    Deinococcus radiodurans, Nicotiana benthamiana
  • Mutation
    N4Y/R110K/V192L/L222I
  • Tag / Fusion Protein
    • AtFDnls (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositing lab recommends using DH10B cells for growth and expression. Please visit https://doi.org/10.64898/2025.12.05.692691 for bioRxiv preprint

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4784 TRV2-eTnpBc-reRNA4PDS was a gift from Savithramma Dinesh-Kumar (Addgene plasmid # 251816 ; http://n2t.net/addgene:251816 ; RRID:Addgene_251816)

  • For your References section:

    High-efficiency, transgene-free plant genome editing by viral delivery of an engineered TnpB. Ugrappa N, Rodriguez JE, Nguyen T, Weissman RF, Thornton BW, Terrace CI, Savage DF, Dinesh-Kumar SP. Nat. Plants (2026). 20 February 2026. 10.1038/s41477-026-02237-4
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