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CBS-3593
(Plasmid #255700)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 255700 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    p15A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SuCas12a2 (no His-tag) and Su-repeat CAO1 guide crRNA
  • Species
    Other
  • Mutation
    N/A
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CBS-3593 was a gift from Chase Beisel (Addgene plasmid # 255700 ; http://n2t.net/addgene:255700 ; RRID:Addgene_255700)
  • For your References section:

    Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA. Dmytrenko O, Neumann GC, Hallmark T, Keiser DJ, Crowley VM, Vialetto E, Mougiakos I, Wandera KG, Domgaard H, Weber J, Gaudin T, Metcalf J, Gray BN, Begemann MB, Jackson RN, Beisel CL. Nature. 2023 Jan;613(7944):588-594. doi: 10.1038/s41586-022-05559-3. Epub 2023 Jan 4. 10.1038/s41586-022-05559-3 PubMed 36599979