CBS-3593
(Plasmid
#255700)
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PurposeSuCas12a2 and gRNA expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 255700 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonep15A
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSuCas12a2 (no His-tag) and Su-repeat CAO1 guide crRNA
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SpeciesOther
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MutationN/A
- Promoter T7
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CBS-3593 was a gift from Chase Beisel (Addgene plasmid # 255700 ; http://n2t.net/addgene:255700 ; RRID:Addgene_255700) -
For your References section:
Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA. Dmytrenko O, Neumann GC, Hallmark T, Keiser DJ, Crowley VM, Vialetto E, Mougiakos I, Wandera KG, Domgaard H, Weber J, Gaudin T, Metcalf J, Gray BN, Begemann MB, Jackson RN, Beisel CL. Nature. 2023 Jan;613(7944):588-594. doi: 10.1038/s41586-022-05559-3. Epub 2023 Jan 4. 10.1038/s41586-022-05559-3 PubMed 36599979